酵母裂解酶Zymolyase® 是通过藤黄节杆菌深层培养获得的酶制剂。它能有效的裂解活酵母细胞细胞壁，产生原生质体或原生质球。其中主要负责裂解活酵母细胞的酶成分是ß-1,3- 葡聚糖昆布五糖水解酶，通过对ß-1,3- 葡聚糖连接位水解葡萄糖聚合物。我们提供两种包装Zymolyase-20T 和Zymolyase-100T。Zymolyase-20T 经过硫酸铵沉淀，Zymolyase-100T 经过亲和色谱法进一步纯化制备。裂解活性取决于酵母菌株、酵母生长阶段或培养条件(6-8)。更多信息下方参考文献提供(12-16)。
[ 反应混合物] 酶溶液: 1ml(0.05-0.1mg/mlforZymolyase®-20T) (0.012-0.024mg/mlforZymolyase®-100T) 啤酒酵母细胞悬液: 3ml (2 mg/ml) 1/15M 磷酸盐缓冲液 :5 ml (pH7.5) 蒸馏水:1 ml
参考文献
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